Geeni N (28274..29533) COVID-19, ORF9 N proteiini , nukleokapsidin rakenneproteiini, 419 aminohappoa

 Geeni "N", ORF9
https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2697049
https://www.ncbi.nlm.nih.gov/nuccore/1798174254
Edelliset  tekijät ovat   viruksen teoreettinen strategia
Nyt seuraa viruksen  todellisuuteen ilmenevä  muoto.
 S, M, E ja N, jotka muovaavat virionin, viruksen  aineellisen  lisääntymiskykyisen osuuden. Joitain työkaluja tietysti tulee  virioneihin mukaan sattumamääriä.

N proteiini, ORF9 ( en näe tässä cysteiiniä C mukana, mutta runsaasti Q glutamiinia)
 https://www.ncbi.nlm.nih.gov/protein/QIA20052.1

 gene            28274..29533
                     /gene="N"
                     /locus_tag="GU280_gp10"
                     /db_xref="GeneID:43740575"
     CDS             28274..29533
                     /gene="N"
                     /locus_tag="GU280_gp10"
                     /note="ORF9; structural protein"
                     /codon_start=1
                     /product="nucleocapsid phosphoprotein"
                     /protein_id="YP_009724397.2"
                     /db_xref="GeneID:43740575"
                     /translation="MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQG
                     LPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMK
                     DLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQ
                     LPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAA
                     LALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGR
                     RGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYT
                     GAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTV
                     TLLPAADLDDFSKQLQQSMSSADSTQA"

Coronavirus nucleocapsid protein is an RNA chaperone.

Zúñiga S, Sola I, Moreno JL, Sabella P, Plana-Durán J, Enjuanes L.

Virology. 2007 Jan 20;357(2):215-27. Epub 2006 Sep 18.

Abstract

RNA chaperones are nonspecific nucleic acid binding proteins with long disordered regions that help RNA molecules to adopt its functional conformation. Coronavirus nucleoproteins (N) are nonspecific RNA-binding proteins with long disordered regions. Therefore, we investigated whether transmissible gastroenteritis coronavirus (TGEV) N protein was an RNA chaperone. Purified N protein enhanced hammerhead ribozyme self-cleavage and nucleic acids annealing, which are properties that define RNA chaperones. In contrast, another RNA-binding protein, PTB, did not show these activities. N protein chaperone activity was blocked by specific monoclonal antibodies. Therefore, it was concluded that TGEV N protein is an RNA chaperone. In addition, we have shown that purified severe acute respiratory syndrome (SARS)-CoV N protein also has RNA chaperone activity. In silico predictions of disordered domains showed a similar pattern for all coronavirus N proteins evaluated. Altogether, these data led us to suggest that all coronavirus N proteins might be RNA chaperones.

PMID:

16979208

DOI:

10.1016/j.virol.2006.07.046

[Indexed for MEDLINE]

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