ilmeinen alkuperä on sittenkin lepakoissa eikä esim käärmeessä. Ei ole kysymys kuitenkaan uudesta mosaiikkaviruksesta, vaan on analysoitavissa fylogeneettista pohjaa vanhoistta tutuista lepakkoviruksistakin, mutta keskimmäisessä genomisessa pätkässä on jotain vielä selvittämätöntä taustaa , mikä ei lähimmistä lepakkoviruksista selkiä. tämä uutuus koskee mm. S-proteiinia osaksi ja ihmisen oireiston taustan selvittelyssä sillä onkin suuri merkitys. Rokotteen kehittelyllä on sikäli tosi kiirettä. koska rokotteella juuri saadaan vastavoimia virionin pinnan S-proteiinin vaikutuksiin . enemmän kuin virusgenomin sisimmän partikkelin osasiin. Niiden suhteen merkitsee strategioitten luomineen. virusmäärän vähentämiseksi ja ottaa aikansa löytää niistä rokotteeseen sopivaa kohtaa.
Infect Genet Evol. 2020 Apr;79:104212. doi: 10.1016/j.meegid.2020.104212. Epub 2020 Jan 29.
Infect Genet Evol. 2020 Apr;79:104212. doi: 10.1016/j.meegid.2020.104212. Epub 2020 Jan 29.
Full-genome evolutionary analysis of the novel corona virus (2019-nCoV) rejects the hypothesis of emergence as a result of a recent recombination event.
Abstract BACKGROUND:
A novel coronavirus (2019-nCoV)
associated with human to human transmission and severe human infection
has been recently reported from the city of Wuhan in China. Our
objectives were to characterize the genetic relationships of the 2019-nCoV and to search for putative recombination within the subgenus of sarbecovirus.METHODS: Putative recombination was investigated by RDP4 and Simplot v3.5.1 and discordant phylogenetic clustering in individual genomic fragments was confirmed by phylogenetic analysis using maximum likelihood and Bayesian methods.
RESULTS: Our analysis suggests that the 2019-nCoV although closely related to BatCoV RaTG13 sequence throughout the genome (sequence similarity 96.3%), shows discordant clustering with the Bat_SARS-like coronavirus sequences.
Specifically, in the 5'-part spanning the first 11,498 nucleotides and the last 3'-part spanning 24,341-30,696 positions, 2019-nCoV and RaTG13 formed a single cluster with Bat_SARS-like coronavirus sequences, whereas in the middle region spanning the 3'-end of ORF1a, the ORF1b and almost half of the spike regions, 2019-nCoV and RaTG13 grouped in a separate distant lineage within the sarbecovirus branch.
CONCLUSIONS:The levels of genetic similarity between the 2019-nCoV and RaTG13 suggest that the latter does not provide the exact variant that caused the outbreak in humans, but the hypothesis that 2019-nCoV has originated from bats is very likely. We show evidence that the novel coronavirus (2019-nCov) is not-mosaic consisting in almost half of its genome of a distinct lineage within the betacoronavirus. These genomic features and their potential association with virus characteristics and virulence in humans need further attention.
Copyright © 2020 Elsevier B.V. All rights reserved. KEYWORDS:
Genomic sequence analysis; Molecular epidemiology; Novel coronavirus; Origin; Phylogenetic analysis; Recombination
- PMID:32004758
- DOI:10.1016/j.meegid.2020.104212
The family Coronaviridae includes a large number of viruses that in nature are found in birds and mammals (Kahn and McIntosh, 2005; Fehr and Perlman, 2015).
Human coronaviruses, first characterized in the 1960s, are associated
with a large percentage of respiratory infections both in children and
adults (Kahn and McIntosh, 2005; Paules et al., 2020).
Scientific
interest in Coronaviruses exponentially increased after the emergence
of SARS-Coronavirus (SARS-CoV) in Southern China (Drosten et al., 2003; Ksiazek et al., 2003; Peiris et al., 2003). Its rapid spread led to the global appearance of more than 8000 human cases and 774 deaths (Kahn and McIntosh, 2005). The virus was initially detected in Himalayan palm civets (Guan et al., 2003)
that may have served as an amplification host; the civet virus
contained a 29-nucleotide sequence not found in most human isolates that
were related to the global epidemic (Guan et al., 2003).
It has been speculated that the function of the affected open reading
frame (ORF 10) might have played a role in the trans-species jump (Kahn and McIntosh, 2005). A similar virus was found later in horseshoe bats (Lau et al., 2005; Li et al., 2005a).
A 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not found in most
human SARS-CoV genomes, was suggestive of a common ancestor with civet
SARS-CoV (Lau et al., 2005).
After the SARS epidemic, bats have been considered as a potential
reservoir species that could be implicated in future coronavirus-related
human pandemics (Cui et al., 2019).
During 2012 Middle East Respiratory coronavirus (MERS-CoV) emerged in Saudi Arabia (Zaki et al., 2012; Hajjar et al., 2013) and has since claimed the lives of 919 out of 2521 (35%) people affected (ECDC, 2020). A main role in the transmission of the virus to humans has been attributed to dromedary camels (Alagaili et al., 2014) and its origin has been again traced to bats (Ithete et al., 2013).
Ever
since both SARS and MERS-CoV (due to their high case fatality rates)
are prioritized together with “highly pathogenic coronaviral diseases
other than MERS and SARS” under the Research and Development Blueprint
published by the WHO (World Health Organization, 2018).
A
novel coronavirus (2019-nCoV) associated with human to human
transmission and severe human infection has been recently reported from
the city of Wuhan in Hubei province in China (World Health Organization, 2020; Hui et al., 2020).
A total of 1,320 confirmed and 1,965 suspect cases were reported up to
25 January 2020; of the confirmed cases 237 were severely ill and 41 had
died (World Health Organization, 2020). Most of the original cases had close contact with a local fresh seafood and an animal market (Zhu et al., 2020; Perlman, 2020).
Full-genome
sequence analysis of 2019-nCoV revealed that belongs to
betacoronavirus, but it is divergent from SARS-CoV and MERS-CoV that
caused epidemics in the past (Zhu et al., 2020). The 2019-nCoV along with the Bat_SARS-like coronavirus forms a distinct lineage within the subgenus of the sarbecovirus (Zhu et al., 2020).
Our
objectives were to characterize the genetic relationships of the
2019-nCoV and to search for putative recombination within the subgenus
of sarbecovirus.
Viral sequences were downloaded from NCBI nucleotide sequence database (http://www.ncbi.nlm.nih.gov). The BatCoV RaTG13 sequence was downloaded from the GISAID BetaCov 2019–2020 repository (http://www.GISAID.org). The sequence was reported in Zhou et al. (2020). Full-genomic sequence alignment was performed using MAFFT v7.4.2. (Katoh and Standley, 2013) and manually edited using MEGA v1.0 (Stecher et al., 2020) according to the encoded reading frame. Putative recombination was investigated by RDP4 (Martin, 2015) and Simplot v3.5.1 (Lole et al., 1999)
and discordant phylogenetic clustering in individual genomic fragments
was confirmed by phylogenetic analysis using maximum likelihood (ML) and
Bayesian methods. ML trees were reconstructed using Neighbor-Joining
(NJ) with ML distances or after heuristic ML search (TBR) with GTR + G
as nucleotide substitution model as implemented in PAUP* 4.0 beta (Swofford, 2003). The GTR + G was used in Bayesian analysis as implemented in MrBayes v3.2.7 (Huelsenbeck and Ronquist, 2001). Phylogenetic trees were viewed using FigTree v1.4 (http://tree.bio.ed.ac.uk/software/figtree/).
A
similarity plot was performed using a sliding window of 450 nts moving
in steps of 50 nts, between the query sequence (2019-nCoV) and different
sequences grouped according to their clustering pattern. The similarity
plot (Fig. 1A,B)
suggested that the RaTG13 was the most closely related sequence to the
2019-nCoV throughout the genome. The genetic similarity between the
2019-nCoV and RaTG13 was 96.3% (p-distance: 0.0369). On the other hand, a
discordant relationship was detected between the query and the
sequences of the Bat_SARS-like coronavirus (MG772934 and MG772933) (Fig. 1C).
Specifically in in the 5′-part of the genome spanning the first 10,901
nts of the alignment that correspond to the 11,498 nucleotides of the
prototype strain (NC_045512) and the last 3′-part spanning 22,831–27,933
positions (24,341–30,696 nucleotides in the NC_045512), 2019-nCoV and
RaTG13 formed a single cluster with Bat_SARS-like coronavirus sequences (Fig. 1C).
In the middle region spanning the 3′-end of ORF1a, the ORF1b and almost
half of the spike regions (10,901–22,830 nts in the alignment or
11,499–24,340 of the NC_045512), 2019-nCoV and RaTG13 grouped in a
separate distant lineage within the sarbecovirus branch (Fig. 1B,
C). In this region the 2019-nCoV and RaTG13 is distantly related to the
Bat_SARS-like coronavirus sequences. Phylogenetic analyses using
different methods confirmed these findings. A BLAST search of 2019-nCoV
middle fragment revealed no considerable similarity with any of the
previously characterized corona viruses (Fig. 2).
Fig. 1.
A. Genomic organization of the novel coronavirus (2019-nCoV) according
to the positions in the edited alignment. B. Simplot of 2019-nCoV
(NC_045512_Wuhan_Hu-1) against sequences within the subgenus
sarbecovirus. Different colours correspond to the nucleotide similarity
between the 2019-nCoV and different groups. The regions with discordant
phylogenetic clustering of the 2019-nCoV with Bats_SARS-like sequences
are shown in different colours. C. Maximum likelihood (ML) phylogenetic
trees inferred in different genomic regions as indicated by the Simplot
analysis. The genomic regions are shown in numbers at the top or at the
left of the trees. The 2019-nCoV sequence is shown in red and stars
indicate important nodes received 100% bootstrap and 1 posterior
probability support. (For interpretation of the references to colour in
this figure legend, the reader is referred to the web version of this
article.)
Our study
suggests that the new corona virus (2019-nCoV) is not a mosaic and it is
most closely related with the BatCoV RaTG13 detected in bats from
Yunnan Province (Zhou et al., 2020).
The levels of genetic similarity between the 2019-nCoV and RaTG13
suggest that the latter does not provide the exact variant that caused
the outbreak in humans, but the hypothesis that 2019-nCoV has originated
from bats is very likely. On the other hand, there is evidence for
discordant phylogenetic relationships between 2019-nCoV and RaTG13 clade
with their closest partners, the Bat_SARS-like coronavirus sequences.
In accordance with previous analysis (http://virological.org/t/ncovs-relationship-to-bat-coronaviruses-recombination-signals-no-snakes/331),
Bat_SARS-like coronavirus sequences cluster in different positions in
the tree, suggesting that they are recombinants, and thus that the
2019-nCoV and RaTG13 are not (Ji et al., 2020; Magiorkinis et al., 2004).
One previous study based on codon usage analyses suggested that the
spike protein of 2019-nCoV might have originated from one yet-unknown
unsampled coronavirus through recombination (Ji et al., 2020).
Codon usage analyses can resolve the origin of proteins with deep
ancestry and insufficient phylogenetic signal or invented de novo. The
recently-published bat coronavirus sequence however provides strong
phylogenetic information to resolve the origin of the Spike protein, as
well as the rest of the genome, suggesting a uniform ancestry across the
genome. We have previously shown that phylogenetic discordance in deep
relationships of coronaviruses is common and can be explained either by
ancient recombination event or altered evolutionary rates in different
lineages, or a combination of both (Magiorkinis et al., 2004).
Our study rejects the hypothesis of emergence as a result of a recent
recombination event.
Notably, the new coronavirus provides a new lineage
for almost half of its genome, with no close genetic relationships to
other viruses within the subgenus of sarbecovirus. This genomic part
comprises half of the spike region encoding a multifunctional protein
responsible also for virus entry into host cells (Babcock et al., 2004; Li et al., 2005b).
The unique genetic features of 2019-nCoV and their potential
association with virus characteristics and virulence in humans remain to
be elucidated.
Declaration of Competing Interest
All authors report no conflict of interest related to the submitted work.
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