Geeni "N", ORF9
https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2697049
https://www.ncbi.nlm.nih.gov/nuccore/1798174254
Edelliset tekijät ovat viruksen teoreettinen strategia
Nyt seuraa viruksen todellisuuteen ilmenevä muoto.
S, M, E ja N, jotka muovaavat virionin, viruksen aineellisen lisääntymiskykyisen osuuden. Joitain työkaluja tietysti tulee virioneihin mukaan sattumamääriä.
N proteiini, ORF9 ( en näe tässä cysteiiniä C mukana, mutta runsaasti Q glutamiinia)
https://www.ncbi.nlm.nih.gov/protein/QIA20052.1
gene 28274..29533 /gene="N" /locus_tag="GU280_gp10" /db_xref="GeneID:43740575" CDS 28274..29533 /gene="N" /locus_tag="GU280_gp10" /note="ORF9; structural protein" /codon_start=1 /product="nucleocapsid phosphoprotein" /protein_id="YP_009724397.2" /db_xref="GeneID:43740575" /translation="MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQG LPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMK DLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAA LALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGR RGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYT GAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTV TLLPAADLDDFSKQLQQSMSSADSTQA"
Zúñiga S, Sola I, Moreno JL, Sabella P, Plana-Durán J, Enjuanes L.
Virology. 2007 Jan 20;357(2):215-27. Epub 2006 Sep 18.
Abstract
RNA chaperones are
nonspecific nucleic acid binding proteins with long disordered regions
that help RNA molecules to adopt its functional conformation. Coronavirus nucleoproteins (N)
are nonspecific RNA-binding proteins with long disordered regions.
Therefore, we investigated whether transmissible gastroenteritis coronavirus (TGEV) N protein was an RNA chaperone. Purified N protein
enhanced hammerhead ribozyme self-cleavage and nucleic acids annealing,
which are properties that define RNA chaperones. In contrast, another
RNA-binding protein, PTB, did not show these activities. N protein chaperone activity was blocked by specific monoclonal antibodies. Therefore, it was concluded that TGEV N protein is an RNA chaperone. In addition, we have shown that purified severe acute respiratory syndrome (SARS)-CoV N protein also has RNA chaperone activity. In silico predictions of disordered domains showed a similar pattern for all coronavirus N proteins evaluated. Altogether, these data led us to suggest that all coronavirus N proteins might be RNA chaperones.
- PMID:
- 16979208
- DOI:
- 10.1016/j.virol.2006.07.046
- [Indexed for MEDLINE]
