SARS-2 CoV nsp 8 proteiinin todettuja interaktio partnereita ihmisen proteiinein joukossas. ( 24)

LISTA  SARS-2 CoV  nsp8  proteiini-proteiini interaktioista ihmisen proteiinien kanssa. Seuraavat  24 proteiinia ( geenit niistä  luetteloitu) on todettu.
 Apuna  PubMed Gerne ja GeneCards^®: The Human Gene Database

 Swiss model  antaa  nsp8 tekijästä monomeerisiä  hahmoja, mutta koska nsp on octameeri ja muodostaa nsp7:n kanssa  hexadecameerin,  monomeerit eivät ole käytännössä relevantteja. 

"Non-structural protein 8 (nsp8): Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. May be required to activate RNA-synthesizing activity of Pol.  Experimental structures (PDB: 6m71, 6wiq, 6wqd, 6yhu, 7btf, 7bv1, 7bv2) and high quality models are available. Monomer models may not be biologically relevant as nsp8 may form a hexadecamer with nsp7 or form a complex with nsp7/Pol - see hetero section".

SARS-2-COV nsp8 proteiini- proteiini-interaktiot (24) 

1. MRPS5,Q11.1) Recommended name: 28S ribosomal protein S5, mitochondrial.https://www.genecards.org/cgi-bin/carddisp.pl?gene=MRPS5&keywords=mrps5
2. SEPSECS,(4p15.2).,  Selenocysteinyl tRNA https://www.genecards.org/cgi-bin/carddisp.pl?gene=SEPSECS&keywords=SEPSECS . The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
3. ATE1, 10q26.13) ,post translational  arginyl ttransferase. https://www.genecards.org/cgi-bin/carddisp.pl?gene=ATE1&keywords=ATE1
4. MRPS27 (5Q13.2) , 28S ribosomal protein S27, mitochondrial, https://www.genecards.org/cgi-bin/carddisp.pl?gene=MRPS27&keywords=MRPS27
   
5. MPHOSPH1, (KIF20B ) (10q23.31)
   (Kinesin Family Member 20B) is a Protein Coding gene. Diseases associated with KIF20B include Bladder Cancer and Primary Autosomal Recessive Microcephaly. Among its related pathways are Golgi-to-ER retrograde transport and Gastric Cancer Network 1. Gene Ontology Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436). KI20B_HUMAN,Q96Q8
6. EXOSC3 ,(9p13.2), Ribosomal RNA-Processing Protein 40 ^{3 4}, Exosome Complex Component RRP40. https://www.genecards.org/cgi-bin/carddisp.pl?gene=EXOSC3&keywords=EXOSC3 This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21.
7. MEPCE, (7.q22.1), 7SK snRNA methylphosphate capping enzyme  ,https://www.genecards.org/cgi-bin/carddisp.pl?gene=MEPCE&keywords=MEPCE
   MEPCE (Methylphosphate Capping Enzyme) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include S-adenosylmethionine-dependent methyltransferase activity. An important paralog of this gene is BCDIN3D.
     S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.
8. EXOSC8, (13q13.3), Exosome complex component RRP43.  https://www.genecards.org/cgi-bin/carddisp.pl?gene=EXOSC8&keywords=EXOSC8.  This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species..
9. EXOSC5 ,(19q13.2), Chronic Myelogenous Leukemia Tumor Antigen 28 ^{3 4}, Ribosomal RNA-Processing Protein 46 ^{3 4}, Exosome Complex Component RRP46 ^.  https://www.genecards.org/cgi-bin/carddisp.pl?gene=EXOSC5&keywords=EXOSC5 Among its related pathways are ATP/ITP metabolism and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include RNA binding and exoribonuclease activity. An important paralog of this gene is EXOSC6.
10. NGDN, (4q11.2), neuroguidin,  Centromere accumalated, EIF4E-Binding. Protein https://www.genecards.org/cgi-bin/carddisp.pl?gene=NGDN&keywords=NGDN.
11. LARP7,(4q25), La related protein, https://www.genecards.org/cgi-bin/carddisp.pl?gene=LARP7&keywords=LARP7.  Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
    • LARP7_HUMAN,Q4G0J3
12. EXOSC2, (9q34.12) Exosome complex component RRP4,  https://www.uniprot.org/uniprot/Q13868#function  (Exosome Component 2). Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7.
13. NSD2, (4p16.3)  Histone-lysine N-methyltransferase NSD2. Nuclear Receptor Binding SET Domain Protein 2.  https://www.genecards.org/cgi-bin/carddisp.pl?gene=NSD2&keywords=NSD2.  MMSET ^{3 4}
14. NARS2,(11q14.1),  Asparaginyl-TRNA Synthetase 2, Mitochondria, Probable asparagine--tRNA ligase, mitochondrial , https://www.genecards.org/Search/Keyword?queryString=NARS2, This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24). [provided by RefSeq, Mar 2015]
15. AATF,(17q12), Apoptosis antagonizing transcription factor, BPR2, https://www.genecards.org/cgi-bin/carddisp.pl?gene=AATF&keywords=AATF ,AATF_HUMAN,Q9NY61
16. MRPS2, (9q34.3), 28S ribosomal protein S2, mitochondrial,https://www.genecards.org/cgi-bin/carddisp.pl?gene=MRPS2&keywords=MRPS2
17. MRPS25,3p25.1), 28S ribosomal protein S25, mitochondrial, https://www.genecards.org/cgi-bin/carddisp.pl?gene=MRPS25&keywords=MRPS25
18. SRP19, ( 5q22.2), Signal-recognition-particle 19,  assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19_HUMAN,P09132 Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
19. DDX10, (11q2.3), Probable ATP-dependent RNA helicase DDX10.   DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it may be involved in ribosome assembly. Fusion of this gene and the nucleoporin gene, NUP98, by inversion 11 (p15q22) chromosome translocation is found in the patients with de novo or therapy-related myeloid malignancies. [provided by RefSeq, Jul 2008]
20. SRP72,(4q12).Signal-recognition-particle  subunit 72,  BMFS1.   The  assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.https://www.genecards.org/cgi-bin/carddisp.pl?gene=SRP72&keywords=SRP7
21. SRP54,  (14q13.2), Signal Recognition Particle 54  is a Protein Coding gene.SRP54_HUMAN,P61011 Diseases associated with SRP54 include Neutropenia, Severe Congenital, 8, Autosomal Dominant and Shwachman-Diamond Syndrome 1. Among its related pathways are Gene Expression and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include GTPase activity. An important paralog of this gene is SRPRA.
    Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
22. NOL10, (2p25.1). Recommended name:Nucleolar protein 10. Polyglutamione binding protein 5, https://www.genecards.org/cgi-bin/carddisp.pl?gene=NOL10&keywords=NOL10
23. CCDC86 (11q22),  (Coiled-Coil Domain Containing 86) is a Protein Coding gene. CYCLON,  Cytokine induced proein  with coiled coil. Diseases associated with CCDC86 include Gaucher Disease, Type Ii and Gastrointestinal Adenoma.
24. HECTD1.(14q12), HECT Domain E3 Ubiquitin Protein Ligase 1., is a Protein Coding gene.https://www.genecards.org/cgi-bin/carddisp.pl?gene=HECTD1&keywords=HECTD1 Diseases associated with HECTD1.  Gene Ontology (GO) annotations related to this gene include binding and ubiquitin-protein transferase activit
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure .HECD1_HUMAN,Q9ULT8

12.5. 2020:  Muistiin SARS-2 CoV nsp 8 non strukturellin replikaasista pilkkoutuneen tekijän    proteiini-proteiini interaktioista (ppi)  ihmisen valkuaisaineisiin. Nsp 8 näyttää keskittyneen käyttämään   kaappaamaansa  ribosomaalista  koneistoa.