SARS-2 Spike-proteiinin interaktioprofiilista, Swiss model

https://covid-19.uniprot.org/uniprotkb/P0DTC2

Function

Spike protein S1

attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes

Spike protein S2

mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes

Spike protein S2'

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis

Interaction

subunit

Spike glycoprotein

Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32075877, PubMed:32155444, PubMed:32245784). The resulting peplomers protrude from the virus surface as spikes (By similarity). Interacts with ORF3a protein and ORF7a protein

Spike protein S1

Binds to host ACE2

Interaction

subunit

Spike glycoprotein

Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32075877, PubMed:32155444, PubMed:32245784). The resulting peplomers protrude from the virus surface as spikes (By similarity). Interacts with ORF3a protein and ORF7a protein

Spike protein S1

Binds to host ACE2

Protein-protein interaction databases ComplexPortal CPX-5682 SARS-CoV-2 Spike protein complex IntAct P0DTC2 3 interactors

	1	spike_wcpv	protein	14	EBI-25474821		EBI-25474821	SPIKE
	2	zdhc5_human	protein	1	EBI-2799626		EBI-2799626	Palmitoyltransferase ZDHHC5  (DHHC - type Znf)
	3	goga7_human	protein	1	EBI-4403685		EBI-4403685	Golgin subfamily A member 7
	4	ace2_human	protein	11	EBI-7730807		EBI-7730807	Angiotensin-converting enzyme 2