COVID-1 työkaluissa nsp2 mainitaan mahdollisena isäntäsolun PHB ja PHB2-proteiinien suosijana. PHB2 suosinta olisikin loogista, jos COVID-19 nsp1 hävittää osan ribosomeista isäntäsolun mRNAn ohella. Samalla viruksella on tarvetta tehostaa ribosomitoimintaa, mutta ei kuitenkaan DNA-synteesiä, jolloin PHB2:n suosiminen on ilmeisen loogista. Mainitaan, että COVID-19 nsp2 on keskittynyt tässä asiassa PHB ja PHB2 interaktioon ja mitokondrian suojaamiseen tietyisti viruksen replikaation eduksi. Myöhäisemmässä vaiheesshan eräät muut virustekijät tuhoavat mitokondrian.
Myös HIV-1 viruksesta mainitaan, että se ottaa tätä proteiinia PHB2 ( molempiakin PHB1/PHB2) komplekseihinsa mukaan. Nämä kuuluvat ihmisen mitokondriaalisiin SPFH- superperheen proteiineihin (stomatiini, prohibitiini, flotilliini ja Hflm/c). Tämä PHB2 esiintyy nukleolissa ja säätelee ribosomaalisen RNA:n transkriptiota ja suojaa esim ribosomien 45S ja 18S synteesiä. Samalla sen vaikutus liittyy DNA-synteesin estoon. Tämä mitokondriaalinen SPFH- proteiiniperhe säätelee replikatiivisen soluelämän pituutta. Prohibitiini 2:n muita nimiä ovat B-soluun assosioitunut proteiini (BAP), p22, D-prohibitiini, B-solureseptoriin assosioitunut proteiini BAP37, PNAS-141, estrogeenireseptoriaktiivisuuden repressori. Sillä on vaikutusta myös inflammasomi NLRP3:n säätelyyn. Sitä sijaitsee mitokondrian sisäkalvossa ja se auttaa pitämään yllä mitokondrian funktiota.
Myös HIV-1 viruksesta mainitaan, että se ottaa tätä proteiinia PHB2 ( molempiakin PHB1/PHB2) komplekseihinsa mukaan. Nämä kuuluvat ihmisen mitokondriaalisiin SPFH- superperheen proteiineihin (stomatiini, prohibitiini, flotilliini ja Hflm/c). Tämä PHB2 esiintyy nukleolissa ja säätelee ribosomaalisen RNA:n transkriptiota ja suojaa esim ribosomien 45S ja 18S synteesiä. Samalla sen vaikutus liittyy DNA-synteesin estoon. Tämä mitokondriaalinen SPFH- proteiiniperhe säätelee replikatiivisen soluelämän pituutta. Prohibitiini 2:n muita nimiä ovat B-soluun assosioitunut proteiini (BAP), p22, D-prohibitiini, B-solureseptoriin assosioitunut proteiini BAP37, PNAS-141, estrogeenireseptoriaktiivisuuden repressori. Sillä on vaikutusta myös inflammasomi NLRP3:n säätelyyn. Sitä sijaitsee mitokondrian sisäkalvossa ja se auttaa pitämään yllä mitokondrian funktiota.
- (Myös: PubMed tieto PHB1 proteiinista (17q21.33) PHB1; HEL-215; HEL-S-54e SummaryThis gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA.
- https://www.ncbi.nlm.nih.gov/pubmed/29324904/
- https://www.ncbi.nlm.nih.gov/gene/5245)
- Jokin neurotrooppinen virus voi käyttä tätä isäntäproteiinia hyödyksi . HIV-1 lataa myös tätä komplekseihinsa)
Prohibitin-2 isoform 1 [Homo sapiens]
NCBI Reference Sequence: NP_001138303.1
LOCUS NP_001138303 299 aa linear PRI 04-DEC-2019 DEFINITION prohibitin-2 isoform 1 [Homo sapiens]. ACCESSION NP_001138303 NP_009204 VERSION NP_001138303.1 DBSOURCE REFSEQ: accession NM_001144831.2 KEYWORDS RefSeq; MANE Select. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (residues 1 to 299) AUTHORS Xu Y, Wang J, Xu W, Ding F and Ding W. TITLE Prohibitin 2-mediated mitophagy attenuates renal tubular epithelial cells injury by regulating mitochondrial dysfunction and NLRP3 inflammasome activation JOURNAL Am. J. Physiol. Renal Physiol. 316 (2), F396-F407 (2019) PUBMED 30539655 REMARK GeneRIF: Prohibitin 2-mediated mitophagy attenuates renal tubular epithelial cells injury by regulating mitochondrial dysfunction and NLRP3 inflammasome activation. REFERENCE 2 (residues 1 to 299) AUTHORS Zhang Y, Wang LN, Lin YN, Xing YX, Shi Y, Zhao J, Chen WW and Han B. TITLE The novel long noncoding RNA LOC283070 is involved in the transition of LNCaP cells into androgen-independent cells via its interaction with PHB2 JOURNAL Asian J. Androl. 20 (5), 511-517 (2018) PUBMED 29956684 REMARK GeneRIF: we found that LOC283070 can bind to PHB2 located in the nucleus and inhibit its effect, and this is one of the mechanisms by which LOC283070 is involved in the transition of LNCaP cells into androgen-independent cells REFERENCE 3 (residues 1 to 299) AUTHORS Xiao Y, Zhou Y, Lu Y, Zhou K and Cai W. TITLE PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver JOURNAL Cell Death Dis 9 (2), 160 (2018) PUBMED 29416008 REMARK GeneRIF: PHB2 forms a ternary protein complex with sequestosome 1 (SQSTM1) and LC3, leading to loading of LC3 onto the damaged mitochondria. Publication Status: Online-Only REFERENCE 4 (residues 1 to 299) AUTHORS Taniguchi K, Matsumura K, Kageyama S, Ii H, Ashihara E, Chano T, Kawauchi A, Yoshiki T and Nakata S. TITLE Prohibitin-2 is a novel regulator of p21(WAF1/CIP1) induced by depletion of gamma-glutamylcyclotransferase JOURNAL Biochem. Biophys. Res. Commun. 496 (1), 218-224 (2018) PUBMED 29307834 REMARK GeneRIF: Taken together, the results indicate that PHB2 plays a central role in p21 upregulation following GGCT knockdown and as such may promote deregulated proliferation of cancer cells by suppressing p21. REFERENCE 5 (residues 1 to 299) AUTHORS Zhou Z, Ai H, Li K, Yao X, Zhu W, Liu L, Yu C, Song Z, Bao Y, Huang Y, Wu Y, Zheng L, Sun Y, Wang G, Ma K, Sun L and Li Y. TITLE Prohibitin 2 localizes in nucleolus to regulate ribosomal RNA transcription and facilitate cell proliferation in RD cells JOURNAL Sci Rep 8 (1), 1479 (2018) PUBMED 29367618 REMARK GeneRIF: High PHB2 expression is associated with high ribosomal RNA transcription and facilitation of cell proliferation in rhabdomyosarcoma. Publication Status: Online-Only REFERENCE 6 (residues 1 to 299) AUTHORS Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD and Chen JL. TITLE Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (17), 9543-9548 (2000) PUBMED 10931946 REFERENCE 7 (residues 1 to 299) AUTHORS Montano MM, Ekena K, Delage-Mourroux R, Chang W, Martini P and Katzenellenbogen BS. TITLE An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens JOURNAL Proc. Natl. Acad. Sci. U.S.A. 96 (12), 6947-6952 (1999) PUBMED 10359819 REFERENCE 8 (residues 1 to 299) AUTHORS Coates PJ, Jamieson DJ, Smart K, Prescott AR and Hall PA. TITLE The prohibitin family of mitochondrial proteins regulate replicative lifespan JOURNAL Curr. Biol. 7 (8), 607-610 (1997) PUBMED 9259555 REFERENCE 9 (residues 1 to 299) AUTHORS Ansari-Lari MA, Shen Y, Muzny DM, Lee W and Gibbs RA. TITLE Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination JOURNAL Genome Res. 7 (3), 268-280 (1997) PUBMED 9074930 REFERENCE 10 (residues 1 to 299) AUTHORS Ansari-Lari MA, Muzny DM, Lu J, Lu F, Lilley CE, Spanos S, Malley T and Gibbs RA. TITLE A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13 JOURNAL Genome Res. 6 (4), 314-326 (1996) PUBMED 8723724 COMMENT VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC110322.1 and U47924.1. On Jun 12, 2012 this sequence version replaced NP_009204.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.496782.1, SRR7410570.496788.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000535923.6/ ENSP00000441875.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## FEATURES Location/Qualifiers source 1..299 /organism="Homo sapiens" /db_xref="taxon:9606" /chromosome="12" /map="12p13.31" Protein 1..299 /product="prohibitin-2 isoform 1" /note="B-cell associated protein; repressor of estrogen receptor activity; D-prohibitin; B-cell receptor-associated protein BAP37" /calculated_mol_wt=33165 Site 2 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N-acetylalanine. {ECO:0000244|PubMed:22814378, ECO:0000269|Ref.8}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Region 19..49 /region_name="Necessary for transcriptional repression. {ECO:0000269|PubMed:10359819}" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99623.2)" Region 40..235 /region_name="SPFH_prohibitin" /note="Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily; cd03401" /db_xref="CDD:259799" Site 128 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphotyrosine. {ECO:0000244|PubMed:15592455}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 147 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine. {ECO:0000250|UniProtKB:O35129}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Region 150..174 /region_name="Necessary for transcriptional repression. {ECO:0000269|PubMed:10359819}" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 151 /site_type="phosphorylation" /experiment="experimental evidence, no additional details recorded" /note="Phosphoserine. {ECO:0000244|PubMed:23186163}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 200 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine. {ECO:0000250|UniProtKB:O35129}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 236 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine. {ECO:0000250|UniProtKB:O35129}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 250 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine. {ECO:0000244|PubMed:19608861}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" Site 262 /site_type="acetylation" /experiment="experimental evidence, no additional details recorded" /note="N6-acetyllysine. {ECO:0000250|UniProtKB:O35129}; propagated from UniProtKB/Swiss-Prot (Q99623.2)" CDS 1..299 /gene="PHB2" /gene_synonym="BAP; Bap37; BCAP37; hBAP; p22; PNAS-141; REA" /coded_by="NM_001144831.2:122..1021" /note="isoform 1 is encoded by transcript variant 1" /db_xref="CCDS:CCDS53741.1" /db_xref="GeneID:11331" /db_xref="HGNC:HGNC:30306" /db_xref="MIM:610704" ORIGIN 1 maqnlkdlag rlpagprgmg talklllgag avaygvresv ftvegghrai ffnriggvqq 61 dtilaeglhf ripwfqypii ydirarprki ssptgskdlq mvnislrvls rpnaqelpsm 121 yqrlgldyee rvlpsivnev lksvvakfna sqlitqraqv sllirrelte rakdfslild 181 dvaitelsfs reytaaveak qvaqqeaqra qflvekakqe qrqkivqaeg eaeaakmlge 241 alsknpgyik lrkiraaqni sktiatsqnr iyltadnlvl nlqdesftrg sdslikgkk //
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