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måndag 2 mars 2020

Muistiin eräs uusi nidovirusrakenne Australiasta takavuosina

 Tämä eläintauti  on nimeltään Wobbly possum disease , Eläin on Australian brushtail possum. Sitä taidetaan myös syödä.  Sillä oli uusi nidovirus, arterivirusryhmää.  Kun katsoo  genomia, siinä on yksi ns3 ja yksi ns8. Muistiin vain tästä  esiintymästä.

https://www.semanticscholar.org/paper/Identification-of-a-novel-nidovirus-associated-with-Dunowska-Biggs/7dd0953c873ea7815f2a498eb3bfd9a9f26cb213/figure/0

Fig. 1. Sequencing strategy and organisation of the partial genome of the novel nidovirus. Top: Location of predicted ORFs (boundaries as per GenBank JN116253), ribosomal frameshift site (RSF), the initial NGS contig, overlapping amplicons (1–10), and 50/30 RACE products (primer sequences available on request). Middle: conserved protein domains including 3CLPRO (arterivirus NSP4 peptidase domain, NSP4_PRO), transmembrane (TM), RNAdependent RNA polymerase (RdRp), putative zinc binding domain (ZBD), viral helicase 1 (HEL1), and Nidovirus EndoU-like (NendoU) domains. Bottom: Alignment of the putative ZBD of arteriviruses (abbreviations defined in Fig. 3) with the corresponding sequence of the putative wobbly possum disease virus (WPDV). Arrows point to conservedHis/Cys residues (vanDinten et al., 2000) identified in theWPDV sequence; His/Cys residues identical in all five sequences are shaded.


Metagenomic discovery and co-infection of diverse wobbly possum disease viruses and a novel hepacivirus in Australian brushtail possums

BackgroundAustralian brushtail possums (Trichosurus vulpecula) are an introduced pest species in New Zealand, but native to Australia where they are protected for biodiversity conservation. Wobbly possum disease (WPD) is a fatal neurological disease of Australian brushtail possums described in New Zealand populations that has been associated with infection by the arterivirus (Arteriviridae) wobbly possum disease virus (WPDV-NZ). Clinically, WPD-infected possums present with chronic… CONTINUE READING

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