GISAID linkki Covid-19 aiheuttajaviruksesta Sars-Cov-2 . Sitaatti.

https://gisaid.org/wiv04/

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hCoV-19/Wuhan/WIV04/2019 (WIV04) is the official reference sequence employed by GISAID (EPI_ISL_402124). WIV04 was chosen because of its high-quality genome sequence and because it represented the consensus of a handful of early submissions for the betacoronavirus responsible for COVID-19. (Pilailuk et al 2020)

WIV04 is representative of and identical to the early outbreak sequences. WIV04 was isolated by the Wuhan Institute of Virology from a clinical sample of a bronchoalveolar lavage fluid (BALF) collected at the Wuhan Jinyintan Hospital in Hubei Province on 30^th December 2019 from a symptomatic patient, a retailer working at the Huanan Seafood Wholesale Market.

BALF was used for RNA extraction and metagenomic next-generation sequencing (NGS). The consensus sequence was obtained by de-novo assembly. (Zheng-Li Shi et al 2020)

ORF1ab

ORF1a

S

ORF3a

ORF3b

E

M

ORF6

ORF7a

ORF7b

ORF8

N

ORF9a

ORF9b

ORF10*

Download FASTA

ORF1ab

Position 266 to 21555, length: 21289nt
(ribosomal frameshift at position 13468)

Proteins

1. ORF1ab polyprotein
   7096 AA
2. Protein by proteolytic cleavage: Nonstructural protein NSP1
   180 AA (1Met-Gly180)
3. Protein by proteolytic cleavage: Nonstructural protein NSP2
   638 AA (181Ala-Gly818)
4. Protein by proteolytic cleavage: Nonstructural protein NSP3
   1944 AA (819Ala-Gly2763)
5. Protein by proteolytic cleavage: Nonstructural protein NSP4
   499 AA (2764Lys-Gln3263)
6. Protein by proteolytic cleavage: Nonstructural protein NSP5
   605 AA (3264Ser-Gln3569)
7. Protein by proteolytic cleavage: Nonstructural protein NSP6
   289 AA (3570Ser-Gln3859)
8. Protein by proteolytic cleavage: Nonstructural protein NSP7
   82 AA (3860Ser-Gln3942)
9. Protein by proteolytic cleavage: Nonstructural protein NSP8
   197 AA (3943Ala-Gln4140)
10. Protein by proteolytic cleavage: Nonstructural protein NSP9
    112 AA (4141Asn-Gln4253)
11. Protein by proteolytic cleavage: Nonstructural protein NSP10
    138 AA (4254Ala-Gln4392)
12. Protein by proteolytic cleavage: Nonstructural protein NSP12
    931 AA (4393Ser-Gln5324)
13. Protein by proteolytic cleavage: Nonstructural protein NSP13
    600 AA (5325Ala-Gln5925)
14. Protein by proteolytic cleavage: Nonstructural protein NSP14
    526 AA (5926Ala-Gln6452)
15. Protein by proteolytic cleavage: Nonstructural protein NSP15
    345 AA (6453Ser-Gln6798)
16. Protein by proteolytic cleavage: Nonstructural protein NSP16
    297 AA (6799Ser-Asn7096)

Evidence in the Literature

1. Denisa Bojkova KK, Koch B, Widera M, Krause D, Ciesek S, Cinatl J, Münch C. SARS-CoV-2 infected host cell proteomics reveal potential therapy targets. Nature (2020) DOI: 10.21203/rs.3.rs-17218/v1
2. Davidson, A.D., Williamson, M.K., Lewis, S. et al. Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein. Genome Med 12, 68 (2020) DOI: 10.1186/s13073-020-00763-0
3. Wu, F., Zhao, S., Yu, B. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020) DOI: 10.1038/s41586-020-2008-3

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