J Gen Virol. 1999 Feb;80 ( Pt 2):355-62.
Characterization of the L gene and 5' trailer region of Ebola virus.
Abstract
The
nucleotide sequences of the L gene and 5' trailer region of Ebola virus
strain Mayinga (subtype Zaire) have been determined, thus completing
the sequence of the Ebola virus genome. The putative transcription
start signal of the L gene was identical to the determined 5' terminus
of the L mRNA (5' GAGGAAGAUUAA) and showed a high degree of similarity
to the corresponding regions of other Ebola virus genes. The 3' end of
the L mRNA terminated with 5' AUUAUAAAAAA, a sequence which is distinct
from the proposed transcription
termination signals of other genes. The 5' trailer sequence of the
Ebola virus genomic RNA consisted of 676 nt and revealed a
self-complementary sequence at the extreme end which may play an
important role in virus replication. The L gene contained a single ORF
encoding a polypeptide of 2212 aa. The deduced amino acid sequence
showed identities of about 73 and 44% to the L proteins of Ebola virus
strain Maleo (subtype Sudan) and Marburg virus, respectively. Sequence
comparison studies of the Ebola virus L proteins with several
corresponding proteins of other non-segmented, negative-strand RNA
viruses, including Marburg viruses, confirmed a close relationship
between filoviruses and members of the Paramyxovirinae. The presence of
several conserved linear domains commonly found within L proteins of
other members of the order Mononegavirales identified this protein as
the RNA-dependent RNA polymerase of Ebola virus.
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