ARD5 is an exemplary monoclonal antibody 8MoAb) that specifically binds to human
TIM- 1 on the BED face of the protein at an epitope
that includes the arginine amino acid residues at positions 85 and 86.
The anti-TIM-1 antibodies described herein inhibit TIM- 1 binding to
phosphatidylserine and dendritic cells and can be used to treat or
prevent immunological disorders such as inflammatory and autoimmune
The amino acid sequence of the human TIM-1 protein is shown as:
MHPQVVILSL ILHLADSVAG SVKVGGEAGP SVTLPCHYSG AVTSMCWRGS CSLFTCQNGI
VWTNGTHVT YRKDTRYKLL GDLSRRDVSL TIENTAVSDS GVYCCRVEHR GWFNDMKITV SLEIVPPKVT TTPIVTTVPT VTTVRTSTTV PTTTTVPMTT VPTTTVPTTM SIPTTTTVLT TMTVSTTTSV PTTTSIPTTT SVPVTTTVST FVPPMPLPRQ NHEPVATSPS SPQPAETHPT TLQGAIRREP TSSPLYSYTT DGNDTVTE SS DGLWNNQTQL FLEHSLLTAN TTKGIYAGVC ISVLVLLALL GVIIAKKYFF
KKEVQQLSVS FSSLQIKALQ NAVEKEVQAE DNIYIENSLY
ATD (SEQ ID NO: 1).
This human TIM-1 protein can be used as an immunogen
to prepare anti- human TIM-1 antibodies. Anti-human TIM-1 antibodies
can then be screened to
identify antibodies having the features described herein (e.g., binding
at an epitope that includes arginine amino acid residues at positions
85 and 86 of TIM- 1)."