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lördag 31 december 2011

(6) HIV-1 ja mannoosia sitova lektiini, komplementtil

Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14079-84. Epub 2011 Jul 28. Designed oligomers of cyanovirin-N show enhanced HIV neutralization.


Division of Biology, Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.


Cyanovirin-N (CV-N) is a small, cyanobacterial lectin that neutralizes many enveloped viruses, including human immunodeficiency virus type I (HIV-1). This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. We created obligate CV-N oligomers to determine whether increasing the number of binding sites has an effect on viral neutralization. A tandem repeat of two CV-N molecules (CVN(2)) increased HIV-1 neutralization activity by up to 18-fold compared to wild-type CV-N. In addition, the CVN(2) variants showed extensive cross-clade reactivity and were often more potent than broadly neutralizing anti-HIV antibodies. The improvement in activity and broad cross-strain HIV neutralization exhibited by these molecules holds promise for the future therapeutic utility of these and other engineered CV-N variants.

[PubMed - indexed for MEDLINE]

PMCID: PMC3161612
[Available on 2012/2/23]

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