Inflammasomi NLRP12 säätelee RIG-1 proteiinia TRIM25 ja RNF125 sinkkisormiproteiinien avulla.

 https://www.genecards.org/cgi-bin/carddisp.pl?gene=NLRP12

Genecards tietoa tästä inflammasomista, joka järjestää  RIG-1 RNA-virussensoria  silppuroitumaan unikitiiniproteosomijärjestelmässä..

Aliases for NLRP12 Gene

• GeneCards Symbol: NLRP12 ²
• NLR Family Pyrin Domain Containing 12 ^{2 3 5}
• PYPAF7 ^{2 3 4 5}
• CLR19.3 ^{2 3 5}
• NALP12 ^{3 4 5}
• RNO2 ^{2 3 5}
• PAN6 ^{2 3 5}
• Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 12 ^{2 3}
• NACHT, LRR And PYD Domains-Containing Protein 12 ^{3 4}

• PYRIN-Containing APAF1-Like Protein 7 ^{3 4}
• Regulated By Nitric Oxide ^{3 4}
• Monarch1 ^{2 5}
• RNO ^{3 4}
• NACHT, Leucine Rich Repeat And PYD Containing 12 ²
• Monarch 1 ³
• Monarch-1 ⁴
• FCAS2 ³

Protein details for NLRP12 Gene (UniProtKB/Swiss-Prot)

Protein Symbol:

P59046-NAL12_HUMAN

Recommended name:

NACHT, LRR and PYD domains-containing protein 12

Protein attributes for NLRP12 Gene

Size:1061 amino acidsMolecular mass: 120173 Da

Quaternary structure:Interacts (via pyrin domain) with ASC. Interacts (via pyrin domain) with FAF1 (via UBA domain) (PubMed:21978668). Interacts with MAP3K14; this interaction promotes proteasomal degradation of MAP3K14 (PubMed:17237370). Interacts with NOD2; this interaction promotes degradation of NOD2 through the ubiquitin-proteasome pathway (PubMed:30559449). Interacts with HSPA1A and HSPA8 (PubMed:17947705). Interacts with HSP90AA1 (PubMed:17947705, PubMed:30559449).

 

 Interacts with TRIM25 (alias RNF147, ZNF147, EFP) ; this interaction inhibits DDX58-mediated signaling pathway (  alias RIG-1 sensoritie)  (PubMed:30902577).

 

 Kommentti:  

TRIM 25 on sinkkisormiproteiini, jolla on RING- tyyppinen sinkkisormi (ZNF147, RNF147)  ja tämä suorittaa DDX5 (-proteiinin aktivaation ubikitinoimalla lysiinin K63, mutta tätä aktivaatiota estää  NLRP12. 

 

Lisäksi NLPR12 vaikuttaa, että toinen RING-tyyppinen sinkkisormiproteiini RNF125 (TRAC1)  ubikitinoi  DDX58-proteiinin toiseen lysiiniin K48, jolloin se suuntautuu proteosomaaliseen silppuriin.

Gene Families for NLRP12 Gene

HGNC:Pyrin domain containing

• NLR family

IUPHAR :

• NOD-like receptor family

Protein Domains for NLRP12 Gene

InterPro:

• P-loop_NTPase
• LRR_dom_sf
• Leu-rich_rpt
• DAPIN
• DEATH-like_dom_sf
• See all 9 »

Blocks:

• Leucine-rich repeat signature
• Pyrin domain
• Leucine-rich repeat, ribonuclease inhibitor subtype

 RNF125 proteiinista lisää tietoa. Se säätelee positiivisesti T-solureseptorin signalointiteitä.

• This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012]

GeneCards Summary for RNF125 Gene

RNF125 (Ring Finger Protein 125) is a Protein Coding gene. Diseases associated with RNF125 include Tenorio Syndrome and Overgrowth Syndrome. Among its related pathways are DDX58/IFIH1-mediated induction of interferon-alpha/beta and Innate Immune System. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF166.

UniProtKB/Swiss-Prot Summary for RNF125 Gene

• E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed:15843525, PubMed:17460044, PubMed:17643463, PubMed:26027934, PubMed:26471729, PubMed:25591766, PubMed:27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation (PubMed:17460044, PubMed:26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed:25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed:26027934). Acts as a positive regulator of T-cell activation (PubMed:15843525).

  • RN125_HUMAN,Q96EQ8

Lisätietoa RNF125  ja sen  kaltaisista RING-sinkkisormiproteiineista (RNFs)  proteiineista:

. 2008 Feb 15;410(1):101-11.

doi: 10.1042/BJ20070995.

T-cell regulator RNF125/TRAC-1 belongs to a novel family of ubiquitin ligases with zinc fingers and a ubiquitin-binding domain

Ana Lucia Giannini  ¹ , Yifang Gao, Marie-José Bijlmakers