J Gen Virol. 2017 Jul;98(7):1620-1629. doi: 10.1099/jgv.0.000842. Epub 2017 Jul 15.
The P gene of rodent brain-adapted measles virus plays a critical role in neurovirulence.
Arai T1, Terao-Muto Y1, Uchida S1, Lin C1, Honda T1, Takenaka A1, Ikeda F1, Sato H1, Yoneda M1, Kai C1.
https://www.ncbi.nlm.nih.gov/pubmed/?term=measles+virus+phosphoprotein+P
Abstract
In rare cases, measles virus (MV) in children leads to fatal neurological complications such as primary measles encephalitis, post-acute measles encephalitis, subacute sclerosing panencephalitis and measles
inclusion-body encephalitis. To investigate the pathogenesis of
MV-induced encephalitis, rodent brain-adapted MV strains CAM/RB and
CAMR40 were generated. These strains acquired mutations to adapt to the
rodent brain during 40 passages in rat brain. However, it is still
unknown which genes confer the neurovirulence of MV. We previously
established a rescue system for recombinant MVs possessing the backbone
of wild-type strain HL, an avirulent strain in mice.
In the present study, to identify the genes in CAMR40 that elicit neurovirulence, we generated chimeric recombinant MVs based on strain HL. As a result, recombinant wild-type MV in which the haemagglutinin (H) gene was substituted with that of CAMR40 caused a non-lethal mild disease in mice, while additional substitution of the HL phosphoprotein (P) gene with that of strain CAMR40 caused lethal severe neurological signs comparable to those of CAMR40. These results clearly indicated that, in addition to the H gene, the P gene is required for the neurovirulence of MV CAMR40.
In the present study, to identify the genes in CAMR40 that elicit neurovirulence, we generated chimeric recombinant MVs based on strain HL. As a result, recombinant wild-type MV in which the haemagglutinin (H) gene was substituted with that of CAMR40 caused a non-lethal mild disease in mice, while additional substitution of the HL phosphoprotein (P) gene with that of strain CAMR40 caused lethal severe neurological signs comparable to those of CAMR40. These results clearly indicated that, in addition to the H gene, the P gene is required for the neurovirulence of MV CAMR40.
- PMID:
- 28708054
- DOI:
- 10.1099/jgv.0.000842
- [Indexed for MEDLINE]
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