281 results
Single-Cell Analysis of Host Responses in Bovine Milk Somatic Cells (bMSCs) Following HPAIV Bovine H5N1 Influenza Exposure.
Viruses. 2025 Jun 3;17(6):811. doi: 10.3390/v17060811.
PMID: 40573402
Free PMC article.
Unlike previously described subclinical influenza A virus (IAV) infections in cattle, H5N1
infection induced severe clinical symptoms, including respiratory
distress, mastitis, and abnormal milk production. To understand the host
immune responses and changes, particularly …
Design, Synthesis and In Vivo Evaluation of a Candidate
Fusion Epitopic Construct Vaccine Based on M2e, HA1, HA2, NA and NP
Fragments of the Highly Pathogenic Avian H5N1 Influenza Virus.
Arch Razi Inst. 2024 Aug 1;79(4):849-856. doi: 10.32592/ARI.2024.79.4.849. eCollection 2024 Aug.
PMID: 40256578
Free PMC article.
One highly regarded solution to this problem is to design
and production of recombinant vaccines using the conserved peptide of influenza viruses. A search of international databases yielded the peptide sequence of the M2e fragment of H5N1 viruses isol …
Conformational Variability Prediction of Influenza Virus
Hemagglutinins with Amino Acid Mutations Using Supersecondary Structure
Code.
Methods Mol Biol. 2025;2870:63-78. doi: 10.1007/978-1-0716-4213-9_5.
PMID: 39543031
The deep neural network-based conformational variability
prediction system of protein structures (SSSCPreds) can simultaneously
predict locations of protein flexibility or rigidity and the shapes of
those regions with high accuracy. The sequence flexibility/rigidity map ob …
Proper development of long-lived memory CD4 T cells requires HLA-DO function.
Front Immunol. 2023 Oct 16;14:1277609. doi: 10.3389/fimmu.2023.1277609. eCollection 2023.
PMID: 37908352
Free PMC article.
INTRODUCTION: HLA-DO (DO) is an accessory protein that binds DM for trafficking to MIIC and has peptide
editing functions. DO is mainly expressed in thymic medulla and B
cells. Using biochemical experiments, our lab has discovered that DO has
differential effects on editin …
The heterophilicic epitopes in conserved HA regions of
human and avian influenza viruses can produce antibodies that bound to
kidney tissue.
Microb Pathog. 2023 Dec;185:106331. doi: 10.1016/j.micpath.2023.106331. Epub 2023 Sep 9.
PMID: 37678657
Free article.
The purpose of this study was to analyze the relationship between heterophilic epitopes on H5N1 hemagglutinin (HA) and disease. The monoclonal antibody (mAb) against H5N1 was prepared, mAbs binding to human kidney tissue were screened, and the reactivities of mAbs w …
Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections.
Cell Rep. 2023 Jul 25;42(7):112766. doi: 10.1016/j.celrep.2023.112766. Epub 2023 Jul 7.
PMID: 37421618
Free article.
To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. ...Overall, this study provides proof of concept for a peptide-based sequential immunization strategy fo …
Beyond the state of the art of reverse vaccinology:
predicting vaccine efficacy with the universal immune system simulator
for influenza.
BMC Bioinformatics. 2023 Jun 5;24(1):231. doi: 10.1186/s12859-023-05374-1.
PMID: 37271819
Free PMC article.
When it was first introduced in 2000, reverse vaccinology
was defined as an in silico approach that begins with the pathogen's
genomic sequence. It concludes with a list of potential proteins with a possible, but not necessarily, list of peptide candidates that need …
Next-generation T cell-activating vaccination increases influenza virus mutation prevalence.
Sci Adv. 2022 Apr 8;8(14):eabl5209. doi: 10.1126/sciadv.abl5209. Epub 2022 Apr 6.
PMID: 35385318
Free PMC article.
To determine the potential for viral adaptation to T cell
responses, we probed the full influenza virus genome by next-generation sequencing directly ex vivo from infected mice, in the context of an experimental T cell-based vaccine, an H5N1-based viral vectored vac …
Infiltration of inflammatory macrophages and neutrophils
and widespread pyroptosis in lung drive influenza lethality in nonhuman
primates.
PLoS Pathog. 2022 Mar 10;18(3):e1010395. doi: 10.1371/journal.ppat.1010395. eCollection 2022 Mar.
PMID: 35271686
Free PMC article.
Here we used a unique translational model of lethal H5N1
influenza in cynomolgus macaques that utilizes inhalation of
small-particle virus aerosols to define mechanisms driving lethal
disease. RNA sequencing of lung tissue revealed an intense interferon response wit …
Transcriptomic Profiling of Mouse Mast Cells upon Pathogenic Avian H5N1 and Pandemic H1N1 Influenza a Virus Infection.
Viruses. 2022 Jan 29;14(2):292. doi: 10.3390/v14020292.
PMID: 35215885
Free PMC article.
To gain further insights into the host cellular responses
of mouse mast cells with influenza A virus infection, such as the highly
pathogenic avian influenza A virus H5N1 and the human pandemic influenza A H1N1, we employed high-throughput RNA sequencing to identify …
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