Bioinformatiikkaan perustuva selitys COVID-19 taudin oireitten taustasta

COVID-19: Attacks the 1-Beta Chain of Hemoglobin and Captures the Porphyrin to Inhibit Human Heme Metabolism

https://chemrxiv.org/articles/COVID-19_Disease_ORF8_and_Surface_Glycoprotein_Inhibit_Heme_Metabolism_by_Binding_to_Porphyrin/11938173?fbclid=IwAR2OeC1y2S_ckxXin0GxfMgJfY7mEh0RGgLhOj6-PzdkwdCIPmC5dMHmKko

The novel coronavirus pneumonia (COVID-19) is an infectious acute respiratory infection caused by the novel coronavirus. The virus is a positive-strand RNA virus with high homology to bat coronavirus. In this study, conserved domain analysis, homology modeling, and molecular docking were used to compare the biological roles of certain proteins of the novel coronavirus. The results showed the ORF8 and surface glycoprotein could bind to the porphyrin, respectively. At the same time, orf1ab, ORF10, and ORF3a proteins could coordinate attack the heme on the 1-beta chain of hemoglobin to dissociate the iron to form the porphyrin. The attack will cause less and less hemoglobin that can carry oxygen and carbon dioxide. The lung cells have extremely intense poisoning and inflammatory due to the inability to exchange carbon dioxide and oxygen frequently, which eventually results in ground-glass-like lung images. The mechanism also interfered with the normal heme anabolic pathway of the human body, is expected to result in human disease. According to the validation analysis of these finds, chloroquine could prevent orf1ab, ORF3a, and ORF10 to attack the heme to form the porphyrin, and inhibit the binding of ORF8 and surface glycoproteins to porphyrins to a certain extent, effectively relieve the symptoms of respiratory distress. Since the ability of chloroquine to inhibit structural proteins is not particularly obvious, the therapeutic effect on different people may be different.  
Favipiravir could inhibit the envelope protein and ORF7a protein bind to porphyrin, prevent the virus from entering host cells, and catching free porphyrins. This paper is only for academic discussion, the correctness needs to be confirmed by other laboratories. Due to the side effects and allergic reactions of drugs such as chloroquine, please consult a qualified doctor for treatment details, and do not take the medicine yourself.

 Content (On Line)
1 Introduction 
2 Materials and methods 
2.1. Data set 
2.2. Flow  chart of bioinformatics 
2.3. Analysis of conserved domains 
2.4. Homology modeling
2.5.  Molecular docking technology
2.6.  Protein docking technology
3 Results
3.1.  Viral structural proteins binding porphyrin (S, E, N, but not M)
3.2. Viral non structural proteins bind to the porphyrin ( orf1ab, ORF7a, ORF8) ( but ORF10, ORF3a and ORF6 could not bind heme  or porphyrin)
3.3. Viral non-structural protein attacks the heme on the beta-chain of the hemoglobin (orf1ab, ORF3a, ORF10) 
 3.4. Validation  for the effect of chloroquine (0TX) phosphate
4 Discussion
4.1. The novel CoV originate from ancient virus 
4.2. Higher permability of porphyrins into cell membranes leads to high infections
4.3. The Complexity of individual immunity
4.4. Immune cells are infected and secrete antibodies and viral proteins
4.5.Viral protein infects hemoglobin by the immune hemolysis of red blood cells
5 Conclusion (We suggest that the damage made by the virus to the human body is systemic, and not  confined ( restricted only) to the respiratory system.
References