Ultraääni stimuloinee ebolan soluunmenovaiheessa sitä funktiota mikä on retrovirukselta kaapattu ?

Virol J. 2011 Sep 22;8:446. doi: 10.1186/1743-422X-8-446.

Effect of ultrasound on herpes simplex virus infection in cell culture.

Shintani M¹, Takahashi G, Hamada M, Okunaga S, Iwai S, Yura Y.

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Abstract

BACKGROUND:

Ultrasound has been shown to increase the efficiency of gene expression from retroviruses, adenoviruses and adeno-associated viruses.
 The effect of ultrasound to stimulate cell membrane permeabilization on infection with an oncolytic herpes simplex virus type 1 (HSV-1) was examined.

RESULTS:

Vero monkey kidney cells were infected with HSV-1 and exposed to 1 MHz ultrasound after an adsorption period. The number of plaques was significantly greater than that of the untreated control. A combination of ultrasound and microbubbles further increased the plaque number. Similar results were obtained using a different type of HSV-1 and oral squamous cell carcinoma (SCC) cells. The appropriate intensity, duty cycle and time of ultrasound to increase the plaque number were 0.5 W/cm², 20% duty cycle and 10 sec, respectively.
Ultrasound with microbubbles at an intensity of 2.0 W/cm², at 50% duty cycle, or for 40 sec reduced cell viability.

CONCLUSION:

These results indicate that ultrasound promotes the entry of oncolytic HSV-1 into cells. It may be useful to enhance the efficiency of HSV-1 infection in oncolytic virotherapy.

PMID:

21939524

[PubMed - indexed for MEDLINE]

PMCID:

PMC3189159

Free PMC Article