Rapid detection of all known ebolavirus species by reverse transcription-loop-mediated isothermal amplification (RT-LAMP).
Abstract
Ebola
virus disease (EVD), a highly virulent infectious disease caused by
ebolaviruses, has a fatality rate of 25-90%. Without a licensed
chemotherapeutic agent or vaccine for the treatment and prevention of
EVD, control of outbreaks requires accurate and rapid diagnosis of
cases. In this study, five sets of six oligonucleotide primers targeting
the nucleoprotein gene were designed for specific identification of
each of the five ebolavirus species using reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay. The detection limits of the ebolavirus
species-specific primer sets were evaluated using in vitro transcribed
RNAs.
The detection limit of species-specific RT-LAMP assays for
The RT-LAMP assays were specific for the detection of the respective species of ebolavirus with no cross reaction with other species of ebolavirus and other viral hemorrhagic fever viruses such as Marburg virus, Lassa fever virus, and Dengue virus. The species-specific RT-LAMP assays developed in this study are rapid, sensitive, and specific and could be useful in case of an EVD outbreak.
The detection limit of species-specific RT-LAMP assays for
- Zaire ebolavirus,
- Sudan ebolavirus,
- Taï Forest ebolavirus, and
- Bundibugyo ebolavirus was 256 copies/reaction,
- Reston ebolavirus was 64 copies/reaction,
The RT-LAMP assays were specific for the detection of the respective species of ebolavirus with no cross reaction with other species of ebolavirus and other viral hemorrhagic fever viruses such as Marburg virus, Lassa fever virus, and Dengue virus. The species-specific RT-LAMP assays developed in this study are rapid, sensitive, and specific and could be useful in case of an EVD outbreak.
Copyright © 2017 Elsevier B.V. All rights reserved.
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