Luonnollinen imuunivaste ja HIV-1 . Artikkeleita.

Löytöjä 3.12. 2018

Vuodelta 2008 
https://www.ncbi.nlm.nih.gov/pubmed/18187620

Science. 2008 Feb 15;319(5865):921-6. doi: 10.1126/science.1152725. Epub 2008 Jan 10.

Identification of host proteins required for HIV infection through a functional genomic screen.

Brass AL¹, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ.Abstract

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle.

Further analysis revealed previously unknown roles for

•  retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, 
• a karyopherin (TNPO3) in viral integration, and 
• the Mediator complex (Med28) in viral transcription. 
• Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle.

 This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy.

PMID:

18187620

DOI:

10.1126/science.1152725

[Indexed for MEDLINE]

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