BatCoV RaTG13 sarbeco Betacoroavirus
Infect Genet Evol. 2020 Mar 3:104272. doi: 10.1016/j.meegid.2020.104272. [Epub ahead of print]
Nonstructural proteins NS7b and NS8 are likely to be phylogenetically associated with evolution of 2019-nCoV.
Abstract
The
seventh novel human infecting Betacoronavirus that causes pneumonia
(2019 novel coronavirus, 2019-nCoV) originated in Wuhan, China. The
evolutionary relationship between 2019-nCoV and the other human
respiratory illness-causing coronavirus is not closely related. We
sought to characterize the relationship of the translated proteins of
2019-nCoV with other species of Orthocoronavirinae. A phylogenetic tree
was constructed from the genome sequences. A cluster tree was developed
from the profiles retrieved from the presence and absence of homologs of
ten 2019-nCoV proteins. The combined data were used to characterize the
relationship of the translated proteins of 2019-nCoV to other species
of Orthocoronavirinae. Our analysis reliably suggests that 2019-nCoV is
most closely related to BatCoV RaTG13
and belongs to subgenus Sarbecovirus of Betacoronavirus, together with
SARS coronavirus and Bat-SARS-like coronavirus. The phylogenetic
profiling cluster of homolog proteins of one annotated 2019-nCoV protein
against other genome sequences revealed two clades of ten 2019-nCoV
proteins. Clade 1 consisted of a group of conserved proteins in
Orthocoronavirinae comprising Orf1ab polyprotein, Nucleocapsid protein,
Spike glycoprotein, and Membrane protein. Clade 2 comprised six proteins
exclusive to Sarbecovirus and Hibecovirus. Two of six Clade 2
nonstructural proteins, NS7b and NS8, were exclusively conserved among
2019-nCoV, BetaCoV_RaTG, and BatSARS-like Cov. NS7b and NS8 have
previously been shown to affect immune response signaling in the
SARS-CoV experimental model. Thus, we speculated that knowledge of the
functional changes in the NS7b and NS8 proteins during evolution may
provide important information to explore the human infective property of
2019-nCoV.
Copyright © 2020. Published by Elsevier B.V. KEYWORDS:
2019-nCoV; Novel proteins; Phylogenetic profile; Phylogenetic tree PMID: 32142938 DOI: 10.1016/j.meegid.2020.104272
Free full text
Inga kommentarer:
Skicka en kommentar